COMPATIBLE TANKS
NuSep provides a range of cassettes to suit your needs - no need to replace your current tank!
NB cassettes (10cm wide X 8.5cm high) will fit in all Bio-Rad™ Mini-Protean™ tanks (including tetra and dodeca cells).
NN cassettes (10cm wide X 10cm high) will fit in the Invitrogen™ X-Cell Sure-Lock™ gel apparatus.
NG cassettes (10cm wide X 8.0cm high) fit in all other 10cm tanks:
• GradiGel Mini 4 -Cell
• EC 4- Cell
• Hoefer Tall Mighty Small (SE 280)
• Hoefer Mighty Small II (SE 260/SE 250)
• Hoefer Mini VE
• Daiichi Mini 2-Gel & 6-Gel
• Owl Road Runner, Penguin
• Invitrogen™ Novex® XCell I and II and SureLock™
• Bio-Rad® Mini-PROTEAN® II & 3
• Owl Single Sided Vertical System
• Shelton IBI protein system
BUFFER FORMULATIONS
Running Buffers (10x)
TruSep sample buffer (2x, 10 mL, no reducing agent)
WESTERN BLOTTING PROTOCOL
*Standard Tris-Glycine can be used. Protocol may need to be optimised.
All proteins stains are compatible with nUView precast gels, including:
• Coomassie blue
• Silver and copper stains
• Fluorescent stains
COMPATIBLE RUNNING BUFFERS
A range of buffers can be used with nUView precast gels. Each buffer produces different migration patterns.
• Tris-Glycine (available from NuSep)